A frame-shifted gene, which rescued its function by non-natural start codons and its application in constructing synthetic gene circuits.
Sarkar K, Mukhopadhyay S, Bonnerjee D, Srivastava R, Bagh S
J Biol Eng. 2019 Mar 1;13:20. doi: 10.1186/s13036-019-0151-x. eCollection 2019.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 193777 | pRA1SEGFPTcIfm | Cassette 1: Expresses EGFP under the control of PR promoter, Cassette 2: Expresses frame-shifted CI under the control of PLTetO-1 promoter, pUC origin of replication, Ampicillin selection |
| 193778 | pTC3cIfm | Expresses frame-shifted cI under the control of PLTetO-1 promoter, p15A origin of replication, Chloramphenicol selection |
| 193786 | pDA2ScIfm | Expresses frame-shifted CI under the control of PLTATAA synthetic promoter, carries a synthetic fragment with additional restriction sites, ColE1 origin of replication, Ampicillin selection |
| 193789 | pTA1cI-T3 | Expresses truncated version of lambda CI named T3 under the control of PLTetO-1 promoter, pUC origin of replication, Ampicillin selection |
| 193790 | pRC3EGFP | Expresses EGFP under the control of PR promoter, p15A origin of replication, Chloramphenicol selection |
| 193791 | pRA1EGFP | Expresses EGFP under the control of PR promoter, pUC origin of replication, Ampicillin selection |
| 193792 | pLA2ScIfmTcIfm | Cassette 1: Expresses frame-shifted CI under PLLacO-1 promoter, Cassette 2: Expresses frame-shifted CI under PLTetO-1 promoter, ColE1 origin of replication, Ampicillin selection |