Martin Buck Lab: Genetic tools for purple bacteria
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 193712 | pRPCE | Expresses cytosine deaminase linked deactivated-Cas9 from RK2 origin with RFP-marked sgRNA insertion site and kanR/sacB selection/counterselection |
| 193726 | pRPCN | Expresses Cas9 from RK2 origin with RFP-marked sgRNA insertion site and kanR/sacB selection/counterselection |
| 193727 | pRSFDuet-galK | Contains expression cassette for E.coli galK for use as a counterselection marker in strains lacking galK |
| 193729 | pStABK1 | Start-Stop assembly level 1 vector, BBR1 origin, kanR |