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Bidirectional communication between droplet interface bilayers driven by cell-free quorum sensing gene circuits

Gonzales DT, Suraritdechachai S, Zechner C, Tang T-YD
ChemSystemsChem 2023 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
193624pEXP5-NT/pLux eGFPInducible expression of eGFP by the transcription factor complex LuxR-3OC6 homoserine lactone (HSL).
193625pEXP5-NT/pT7 LuxRConstitutive T7 RNAP-mediated expression of LuxR transcription factor for Lux quorum sensing system. LuxR sequence is codon optimized for E. coli.
193626pEXP5-NT/pT7 LuxIConstitutive T7 RNAP-mediated expression of LuxI 3OC6-HSL synthase for Lux quorum sensing system. LuxI sequence is codon optimized for E. coli.
193627pEXP5-NT/pLux LuxIInducible expression of LuxI by the transcription factor complex LuxR-3OC6 homoserine lactone (HSL). LuxI is codon optimized for E. coli.
193628pEXP5-NT/pLux LasIInducible expression of LasI by the transcription factor complex LuxR-3OC6 homoserine lactone (HSL). LasI is codon optimized for E. coli.
193629pEXP5-NT/pT7 LasRConstitutive T7 RNAP-mediated expression of LasR transcription factor for Las quorum sensing system. LasR sequence is codon optimized for E. coli.
193630pEXP5-NT/pLas eGFPInducible expression of eGFP by the transcription factor complex LasR-3OC12 homoserine lactone (HSL).
193631pEXP5-NT/pT7 LasIConstitutive T7 RNAP-mediated expression of LasI 3OC12-HSL synthase for Las quorum sensing system. LasI sequence is codon optimized for E. coli.

Antibodies from Article