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Lysosomal positioning regulates Rab10 phosphorylation at LRRK2(+) lysosomes.

Kluss JH, Beilina A, Williamson CD, Lewis PA, Cookson MR, Bonet-Ponce L
Proc Natl Acad Sci U S A. 2022 Oct 25;119(43):e2205492119. doi: 10.1073/pnas.2205492119. Epub 2022 Oct 18. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
193582LYSO-LRRK2Expresses human full-length LRRK2 with a lysosomal targeting sequence from LAMTOR1 (1-39aa) and 3xFLAG on the N-terminus
193583EE-LRRK2Expresses full-length LRRK2 with FYVE-CC2 domains from HRS and 3xFLAG on the N-terminus
1935843xFLAG-FKBP-LRRK2Expresses human full-length LRRK2 with FKBP domain and 3xFLAG situated at the N-terminus

Antibodies from Article