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Evolution of giant pandoravirus revealed by CRISPR/Cas9.

Bisio H, Legendre M, Giry C, Philippe N, Alempic JM, Jeudy S, Abergel C
Nat Commun. 2023 Jan 26;14(1):428. doi: 10.1038/s41467-023-36145-4. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
193453vc241Expression of rRFP in A. castellanii
193454vHB8Expression of GFP-Cas9-NLS and gRNA in A. castellanii
193455vHB16Expression of GFP-Cas9-NLS and gRNA in A. castellanii
193456vHB66Vector for expression of second copy genes in pandoravirus/mollivirus
193457vHB1783xHA endogenous tagging of pandoravirus/mollivirus genes
193458vHB1793xFLAG endogenous tagging of pandoravirus/mollivirus genes

Antibodies from Article