Monitoring glycolytic dynamics in single cells using a fluorescent biosensor for fructose 1,6-bisphosphate.
Koberstein JN, Stewart ML, Smith CB, Tarasov AI, Ashcroft FM, Stork PJS, Goodman RH
Proc Natl Acad Sci U S A. 2022 Aug 2;119(31):e2204407119. doi: 10.1073/pnas.2204407119. Epub 2022 Jul 26.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 193447 | CMV-Hylight | A fluorescent biosensor for fructose 1,6-bisphosphate (FBP). Changes in FBP concentration strongly correlate with changes in glycolytic flux. (mammalian expression vector) |
| 193448 | CMV-Hylight-T152E | A binding-dead fluorescent biosensor for fructose 1,6-bisphosphate (FBP) for negative control experiments (mammalian expression vector) |