Closely related type II-C Cas9 orthologs recognize diverse PAMs.
Wei J, Hou L, Liu J, Wang Z, Gao S, Qi T, Gao S, Sun S, Wang Y
Elife. 2022 Aug 12;11. pii: 77825. doi: 10.7554/eLife.77825.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 192143 | Nsp2Cas9_AAV | Expresses Nsp2Cas9, and cloning backbone for sgRNA |
| 192144 | Nsp2-SmuCas9_AAV | Expresses Nsp2-SmuCas9, and cloning backbone for sgRNA |
| 192145 | NarCas9_AAV | Expresses NarCas9?and cloning backbone for sgRNA |
| 192146 | U6-Nme2_tracr | Expresses Nme2-sgRNA cloned with BspQI |