GB_SynP: A Modular dCas9-Regulated Synthetic Promoter Collection for Fine-Tuned Recombinant Gene Expression in Plants.
Moreno-Gimenez E, Selma S, Calvache C, Orzaez D
ACS Synth Biol. 2022 Sep 16;11(9):3037-3048. doi: 10.1021/acssynbio.2c00238. Epub 2022 Aug 31.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 193103 | GB1839 | GB-cassette for the expression of guide RNA targeting the DFR promoter in -376 position (gRNA2), with 2.1 Ms2 aptamer in the 3' of the scaffold. |
| 193104 | GB2566 | Minimal promoter of SlDFR gene containing 62bp upstream the transcription start site and the 5'UTR region. |
| 193105 | GB3408 | Minimal promoter of slCHS1 gene (Solyc09g091510) containing 62bp upstream the transcription start site and the 5'UTR region. |
| 193106 | GB3409 | Minimal promoter of slANS gene (Solyc10g076660) containing 62bp upstream the transcription start site and the 5'UTR region. |
| 193107 | GB3410 | Minimal promoter of NtTA29 gene containing 62bp upstream the transcription start site and the 5'UTR region (from GB1477 sequence). |
| 193108 | GB3411 | Minimal promoter of At2S3 gene containing 84bp upstream the transcription start site and the 5'UTR region (from GB0029 sequence). |
| 193109 | GB3412 | Minimal promoter of NtPCPS2 gene containing 62bp upstream the transcription start site and the 5'UTR region (from GB1027 sequence). |
| 193110 | GB3413 | Minimal promoter of PcPAF gene containing 62bp upstream the transcription start site and the 5'UTR region (from FB029 sequence). |
| 193111 | GB3414 | Minimal promoter of AnGPDA gene containing 62bp upstream the transcription start site and the 5'UTR region (from FB007 sequence). |
| 193112 | GB2815 | Random sequence R1 of 1240 bp for A1 distal promoter position. |
| 193113 | GB3269 | Random sequence R2 of 1240 bp for A1 distal promoter position. |
| 193114 | GB3270 | Random sequence R3 of 1240 bp for A1 distal promoter position. |
| 193115 | GB2878 | A2 Proximal promoter sequence consisting of the target sequences for the gRNA1 (GB1838) and gRNA2 (GB1839) flanked by random sequences. |
| 193116 | GB2879 | A2 Proximal promoter sequence consisting of the target sequences for the gRNA1 (GB1838) and gRNA2 (GB1839) flanked by random sequences. |
| 193117 | GB2880 | A2 Proximal promoter sequence consisting of the target sequences for the gRNA1 (GB1838) and gRNA2 (GB1839) flanked by random sequences. |
| 193118 | GB2881 | A2 Proximal promoter sequence consisting of the target sequences for the gRNA1 (GB1838) and gRNA2 (GB1839) flanked by random sequences. |
| 193119 | GB2882 | A2 Proximal promoter sequence consisting of the target sequences for the gRNA1 (GB1838) and gRNA2 (GB1839) flanked by random sequences. |
| 193120 | GB2883 | A2 Proximal promoter sequence consisting of the target sequences for the gRNA1 (GB1838) and gRNA2 (GB1839) flanked by random sequences. |
| 193121 | GB3271 | A2 Proximal promoter sequence consisting of the target sequences for the gRNA1 (GB1838) and gRNA2 (GB1839) flanked by random sequences. |
| 193122 | GB2884 | A2 Proximal promoter sequence consisting of two times the target sequence for the gRNA1 (GB1838) flanked by random sequences. |
| 193123 | GB2885 | A2 Proximal promoter sequence consisting of two times the target sequence for the gRNA1 (GB1838) flanked by random sequences. |
| 193124 | GB2886 | A2 Proximal promoter sequence consisting of two times the target sequence for the gRNA1 (GB1838) flanked by random sequences. |
| 193125 | GB2887 | A2 Proximal promoter sequence consisting of two times the target sequence for the gRNA1 (GB1838) flanked by random sequences. |
| 193126 | GB2888 | A2 Proximal promoter sequence consisting of two times the target sequence for the gRNA1 (GB1838) flanked by random sequences. |
| 193127 | GB2889 | A2 Proximal promoter sequence consisting of two times the target sequence for the gRNA1 (GB1838) flanked by random sequences. |
| 193128 | GB2891 | A2 Proximal promoter sequence consisting of three times the target sequence for the gRNA1 (GB1838) flanked by random sequences. |
| 193129 | GB3275 | A2 Proximal promoter sequence consisting of three times the target sequence for the gRNA1 (GB1838) flanked by random sequences. |
| 193130 | GB3276 | A2 Proximal promoter sequence consisting of three times the target sequence for the gRNA1 (GB1838) flanked by random sequences. |
| 193131 | GB3277 | A2 Proximal promoter sequence consisting of three times the target sequence for the gRNA1 (GB1838) flanked by random sequences. |
| 193132 | GB3278 | A2 Proximal promoter sequence consisting of three times the target sequence for the gRNA1 (GB1838) flanked by random sequences. |
| 193133 | GB2890 | A2 Proximal promoter sequence consisting of the target sequence for the gRNA1 (GB1838) at "d site" (-120 from TSS) and gRNA2 (GB1839) flanked by random sequences. |
| 193134 | GB3272 | A2 Proximal promoter sequence consisting of the target sequence for the gRNA1 (GB1838) at "b site" (-210 from TSS). |
| 193135 | GB3273 | A2 Proximal promoter sequence consisting of the target sequence for the gRNA1 (GB1838) at "e site" (-320 from TSS). |
| 193136 | GB3274 | A2 Proximal promoter sequence consisting of the target sequences for the gRNA3 (GB1724) and gRNA2 (GB1839) flanked by random sequences. |