Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
GB_SynP: A Modular dCas9-Regulated Synthetic Promoter Collection for Fine-Tuned Recombinant Gene Expression in Plants.
Moreno-Gimenez E, Selma S, Calvache C, Orzaez D
ACS Synth Biol. 2022 Sep 16;11(9):3037-3048. doi: 10.1021/acssynbio.2c00238. Epub 2022 Aug 31. PubMed Article

Plasmids from Article

ID Plasmid Purpose
193103GB1839GB-cassette for the expression of guide RNA targeting the DFR promoter in -376 position (gRNA2), with 2.1 Ms2 aptamer in the 3' of the scaffold.
193104GB2566Minimal promoter of SlDFR gene containing 62bp upstream the transcription start site and the 5'UTR region.
193105GB3408Minimal promoter of slCHS1 gene (Solyc09g091510) containing 62bp upstream the transcription start site and the 5'UTR region.
193106GB3409Minimal promoter of slANS gene (Solyc10g076660) containing 62bp upstream the transcription start site and the 5'UTR region.
193107GB3410Minimal promoter of NtTA29 gene containing 62bp upstream the transcription start site and the 5'UTR region (from GB1477 sequence).
193108GB3411Minimal promoter of At2S3 gene containing 84bp upstream the transcription start site and the 5'UTR region (from GB0029 sequence).
193109GB3412Minimal promoter of NtPCPS2 gene containing 62bp upstream the transcription start site and the 5'UTR region (from GB1027 sequence).
193110GB3413Minimal promoter of PcPAF gene containing 62bp upstream the transcription start site and the 5'UTR region (from FB029 sequence).
193111GB3414Minimal promoter of AnGPDA gene containing 62bp upstream the transcription start site and the 5'UTR region (from FB007 sequence).
193112GB2815Random sequence R1 of 1240 bp for A1 distal promoter position.
193113GB3269Random sequence R2 of 1240 bp for A1 distal promoter position.
193114GB3270Random sequence R3 of 1240 bp for A1 distal promoter position.
193115GB2878A2 Proximal promoter sequence consisting of the target sequences for the gRNA1 (GB1838) and gRNA2 (GB1839) flanked by random sequences.
193116GB2879A2 Proximal promoter sequence consisting of the target sequences for the gRNA1 (GB1838) and gRNA2 (GB1839) flanked by random sequences.
193117GB2880A2 Proximal promoter sequence consisting of the target sequences for the gRNA1 (GB1838) and gRNA2 (GB1839) flanked by random sequences.
193118GB2881A2 Proximal promoter sequence consisting of the target sequences for the gRNA1 (GB1838) and gRNA2 (GB1839) flanked by random sequences.
193119GB2882A2 Proximal promoter sequence consisting of the target sequences for the gRNA1 (GB1838) and gRNA2 (GB1839) flanked by random sequences.
193120GB2883A2 Proximal promoter sequence consisting of the target sequences for the gRNA1 (GB1838) and gRNA2 (GB1839) flanked by random sequences.
193121GB3271A2 Proximal promoter sequence consisting of the target sequences for the gRNA1 (GB1838) and gRNA2 (GB1839) flanked by random sequences.
193122GB2884A2 Proximal promoter sequence consisting of two times the target sequence for the gRNA1 (GB1838) flanked by random sequences.
193123GB2885A2 Proximal promoter sequence consisting of two times the target sequence for the gRNA1 (GB1838) flanked by random sequences.
193124GB2886A2 Proximal promoter sequence consisting of two times the target sequence for the gRNA1 (GB1838) flanked by random sequences.
193125GB2887A2 Proximal promoter sequence consisting of two times the target sequence for the gRNA1 (GB1838) flanked by random sequences.
193126GB2888A2 Proximal promoter sequence consisting of two times the target sequence for the gRNA1 (GB1838) flanked by random sequences.
193127GB2889A2 Proximal promoter sequence consisting of two times the target sequence for the gRNA1 (GB1838) flanked by random sequences.
193128GB2891A2 Proximal promoter sequence consisting of three times the target sequence for the gRNA1 (GB1838) flanked by random sequences.
193129GB3275A2 Proximal promoter sequence consisting of three times the target sequence for the gRNA1 (GB1838) flanked by random sequences.
193130GB3276A2 Proximal promoter sequence consisting of three times the target sequence for the gRNA1 (GB1838) flanked by random sequences.
193131GB3277A2 Proximal promoter sequence consisting of three times the target sequence for the gRNA1 (GB1838) flanked by random sequences.
193132GB3278A2 Proximal promoter sequence consisting of three times the target sequence for the gRNA1 (GB1838) flanked by random sequences.
193133GB2890A2 Proximal promoter sequence consisting of the target sequence for the gRNA1 (GB1838) at "d site" (-120 from TSS) and gRNA2 (GB1839) flanked by random sequences.
193134GB3272A2 Proximal promoter sequence consisting of the target sequence for the gRNA1 (GB1838) at "b site" (-210 from TSS).
193135GB3273A2 Proximal promoter sequence consisting of the target sequence for the gRNA1 (GB1838) at "e site" (-320 from TSS).
193136GB3274A2 Proximal promoter sequence consisting of the target sequences for the gRNA3 (GB1724) and gRNA2 (GB1839) flanked by random sequences.

Antibodies from Article