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Cell-specific regulation of gene expression using splicing-dependent frameshifting.

Ling JP, Bygrave AM, Santiago CP, Carmen-Orozco RP, Trinh VT, Yu M, Li Y, Liu Y, Bowden KD, Duncan LH, Han J, Taneja K, Dongmo R, Babola TA, Parker P, Jiang L, Leavey PJ, Smith JJ, Vistein R, Gimmen MY, Dubner B, Helmenstine E, Teodorescu P, Karantanos T, Ghiaur G, Kanold PO, Bergles D, Langmead B, Sun S, Nielsen KJ, Peachey N, Singh MS, Dalton WB, Rajaii F, Huganir RL, Blackshaw S
Nat Commun. 2022 Oct 1;13(1):5773. doi: 10.1038/s41467-022-33523-2. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
193014pSLED.NPLNeuron-specific reporter (CBh promoter, Pls3 exon, bichromatic reporter)
193015pSLED.RABPhotoreceptor-specific reporter (CBh promoter, Atp1b2 exon, bichromatic reporter)
193016pSLED.ENSExcitatory neuron-specific reporter (hSyn promoter, Synrg exon, bichromatic reporter)
193017pSLED.GluA2GluA2 (Gria2) flip/flop reporter (hSyn promoter, Gria2 flip/flop exons, bichromatic reporter)
193018pSLED.CaRPv1Excitatory/Inhibitory neuron calcium indicator (hSyn promoter, Synrg exon, jRGECO1a/jGCaMP7b)

Antibodies from Article