An evolutionarily-conserved Wnt3/beta-catenin/Sp5 feedback loop restricts head organizer activity in Hydra.
Vogg MC, Beccari L, Iglesias Olle L, Rampon C, Vriz S, Perruchoud C, Wenger Y, Galliot B
Nat Commun. 2019 Jan 18;10(1):312. doi: 10.1038/s41467-018-08242-2.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 192985 | HySp5:Luc | Plasmid to test the Hydra Sp5 promoter activity |
| 192986 | HyWnt3:Luc | Plasmid to test the Hydra Wnt3 promoter activity |
| 192987 | HyWnt3-ΔRep:Luc | Plasmid to test the Hydra Wnt3 promoter activity (full deletion of the repressor element) |
| 192988 | HyWnt3-ΔRep-D1:Luc | Plasmid to test the Hydra Wnt3 promoter activity (partial deletion of the repressor element-D1) |
| 192989 | HyWnt3-ΔRep-D2:Luc | Plasmid to test the Hydra Wnt3 promoter activity (partial deletion of the repressor element-D2) |
| 192990 | HyWnt3-ΔRep-D3:Luc | Plasmid to test the Hydra Wnt3 promoter activity (partial deletion of the repressor element-D3) |
| 192991 | HyWnt3-ΔRep-D4:Luc | Plasmid to test the Hydra Wnt3 promoter activity (partial deletion of the repressor element-D4) |
| 192992 | ZfWnt3:Luc | Plasmid to test the Zebrafish Wnt3 promoter activity |
| 192993 | HySp5-420 | Plasmid that encodes for Hydra Sp5 |
| 192994 | HySp5-337 | Plasmid that encodes for Hydra Sp5, lacking the DNA binding domain |
| 192995 | HySp5-310 | Plasmid that encodes for Hydra Sp5, lacking the SP box |
| 192996 | HySp5-227 | Plasmid that encodes for Hydra Sp5, lacking the SP box and DNA binding domain |
| 192997 | ZfSp5a-377 | Plasmid that encodes for Zebrafish Sp5 |
| 192999 | ZfSp5l1 | Plasmid that encodes for Zebrafish Sp5-like |
| 193000 | pGEM-T-Easy-HySp5-557 | Plasmid to generate an ISH probe for Hydra Sp5 |