An Inducible T7 Polymerase System for High-Level Protein Expression in Diverse Gram-Negative Bacteria.
Greenwich JL, Alakavuklar MA, Fuqua C
Microbiol Resour Announc. 2023 Feb 16;12(2):e0111922. doi: 10.1128/mra.01119-22. Epub 2023 Jan 16.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
192979 | pMAT36 | BHR plasmid (pRA301) expressing mirA-3XFLAG from T7 promoter for high yield protein purification |
192980 | pJLG029 | BHR plasmid (pRA301) expressing pruR-3XFLAG from T7 promoter for high yield protein purification |
192981 | pJLG038 | BHR plasmid (pBBR1 ori) expressing T7 polymerase under control of the lac promoter |
192982 | pJLG039 | BHR plasmid (IncP ori) expressing T7 polymerase under control of the lac promoter |