Monitoring autochthonous lung tumors induced by somatic CRISPR gene editing in mice using a secreted luciferase.
Merle N, Elmshauser S, Strassheimer F, Wanzel M, Konig AM, Funk J, Neumann M, Kochhan K, Helmprobst F, Pagenstecher A, Nist A, Mernberger M, Schneider A, Braun T, Borggrefe T, Savai R, Timofeev O, Stiewe T
Mol Cancer. 2022 Oct 3;21(1):191. doi: 10.1186/s12943-022-01661-2.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 192871 | pShuttle | Gateway compatible vector with BsaI cloning site |
| 192930 | pShuttle.Cre | Gateway compatible vector with BsaI cloning site and Cre expression |
| 192931 | pShuttle.CC9 | Gateway compatible vector with BsaI cloning site and Cre-2A-Cas9 expression |
| 192932 | pShuttle-PR.CC9 | pShuttle.CC9 encoding sgRNAs targeting Trp53 and Rb1 genes |
| 192933 | pShuttle-PR.Cre | pShuttle.Cre encoding sgRNAs targeting Trp53 and Rb1 genes |
| 192934 | pShuttle-PRL.Cre | pShuttle.Cre encoding sgRNAs targeting Trp53, Rb1 and Rbl2 genes |
| 192935 | pAd-PR.CC9 | Adenoviral expression vector encoding sgRNAs targeting Trp53 and Rb1, Cre and Cas9 |
| 192936 | pAd-PR.Cre | Adenoviral expression vector encoding sgRNAs targeting Trp53 and Rb1 and Cre |
| 192937 | pAd-PRL.Cre | Adenoviral expression vector encoding sgRNAs targeting Trp53, Rb1 and Rbl2 and Cre |