Mitoguardin-2-mediated lipid transfer preserves mitochondrial morphology and lipid droplet formation.
Hong Z, Adlakha J, Wan N, Guinn E, Giska F, Gupta K, Melia TJ, Reinisch KM
J Cell Biol. 2022 Dec 5;221(12):e202207022. doi: 10.1083/jcb.202207022. Epub 2022 Oct 25.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 192832 | pET28-6xhis-sumo-CeMIGA(106-496) | Express C.elegans MIGA fragment 106-496 in bacteria |
| 192833 | pCMV10-3xFLAG-prs-hMIGA2 (170-593) | Express codon-optimized human MIGA2 fragment 170-593 in Expi293 cells |
| 192834 | pET29-hMIGA2(170-593)-6xhis | Express codon-optimized human MIGA2 fragment 170-593 with a C-terminal 6xhis tag (lipid transfer construct) in bacteria |
| 192835 | pCMV10-3xFLAG-prs-hMIGA2(170-593)-25GS-6xhis | Express codon-optimized human MIGA2 fragment 170-593 with a GS-linker and 6xhis tag at C-terminus (lipid transfer construct) in Expi293 cells |
| 192837 | pET29-hMIGA2(306-593)-6xhis | Express codon-optimized human MIGA2 fragment 306-593 with a C-terminal 6xhis tag (lipid transfer construct) in bacteria |
| 192866 | pLVX-hMIGA2-DsRed | Express codon-optimized human MIGA2 full length with a C-terminal DsRed tag in mammalian cells |
| 192867 | pGK-hMIGA2-DsRed | Express codon-optimized human MIGA2 full length with a C-terminal DsRed tag in mammalian cells |