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NCOA4 drives ferritin phase separation to facilitate macroferritinophagy and microferritinophagy.

Ohshima T, Yamamoto H, Sakamaki Y, Saito C, Mizushima N
J Cell Biol. 2022 Oct 3;221(10). pii: 213442. doi: 10.1083/jcb.202203102. Epub 2022 Sep 6. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
192800pMRX-IB-mGFP-FTH1Stable expression of mGFP-FTH1 in mammalian cells
192801pMRX-IB-mGFP-FTLStable expression of mGFP-FTL in mammalian cells
192802pMRX-IP-mGFP-NCOA4Stable expression of mGFP-NCOA4 in mammalian cells
192803pMRX-IP-mGFP-TAX1BP1Stable expression of mGFP-TAX1BP1 in mammalian cells
192804pMRX-IB-mRuby3-FTH1Stable expression of mRuby3-FTH1 in mammalian cells
192805pMRX-IB-mRuby3-FTLStable expression of mRuby3-FTL in mammalian cells
192806pMRX-IP-HaloTag7-NCOA4Stable expression of HaloTag7-NCOA4 in mammalian cells
192807pMRX-IP-3xFLAG-NCOA4Stable expression of 3xFLAG-NCOA4 in mammalian cells
192808pMRX-IP-3xFLAG-NCOA4 (dN)Stable expression of 3xFLAH-NCOA4 (delta N) in mammalian cells
192809pMRX-IP-3xFLAG-NCOA4 (dIDR1)Stable expression of 3xFLAH-NCOA4 (delta IDR1) in mammalian cells
192810pMRX-IP-3xFLAG-NCOA4 (dM)Stable expression of 3xFLAH-NCOA4 (delta M) in mammalian cells
192811pMRX-IP-3xFLAG-NCOA4 (dIDR2)Stable expression of 3xFLAH-NCOA4 (delta IDR2) in mammalian cells
192812pMRX-IP-3xFLAG-NCOA4 (dC)Stable expression of 3xFLAH-NCOA4 (delta C) in mammalian cells
192813pMRX-IP-3xFLAG-NCOA4 (I56E)Stable expression of 3xFLAH-NCOA4 (I56E mutant) in mammalian cells
192814pMRX-IP-3xFLAG-NCOA4 (L63R)Stable expression of 3xFLAH-NCOA4 (L63R mutant) in mammalian cells

Antibodies from Article