An Alternate Approach to Generate Induced Pluripotent Stem Cells with Precise CRISPR/Cas9 Tool.
Javaid N, Choi S
Stem Cells Int. 2022 Sep 22;2022:4537335. doi: 10.1155/2022/4537335. eCollection 2022.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
188975 | pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)_g1_CASH-1 | A human codon-optimized SpCas9 nickase and chimeric g1 guide RNA expression plasmid. |
188976 | pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)_g2_CASH-1 | A human codon-optimized SpCas9 nickase and chimeric g2 guide RNA expression plasmid. |
188977 | pTetO-hOSK-CASH-1 | A CASH-1 GSH targeting, doxycycling inducible humanized Oct4, Sox2, and Klf4 expressing CRISPR/Cas9 donor plasmid. |