CRISPR-based VEGF suppression using paired guide RNAs for treatment of choroidal neovascularization.
Chung SH, Sin TN, Dang B, Ngo T, Lo T, Lent-Schochet D, Meleppat RK, Zawadzki RJ, Yiu G
Mol Ther Nucleic Acids. 2022 Apr 27;28:613-622. doi: 10.1016/j.omtn.2022.04.015. eCollection 2022 Jun 14.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 211758 | px552-U6-gRNA1-CMV-eGFP | sgRNA1 targeting Exon 1 of VEGFA gene conserved across mouse, rhesus macaque, and human. |
| 211759 | px552-U6-gRNA2-CMV-eGFP | sgRNA2 targeting Exon 1 of VEGFA gene conserved across mouse, rhesus macaque, and human. |
| 211760 | px552-U6-gRNA1-U6-gRNA2-CMV-eGFP | Paired gRNAs (sgRNA1 and 2) targeting Exon 1 of VEGFA gene conserved across mouse, rhesus macaque, and human. |