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Improved Methods for Deamination-Based m(6)A Detection.

Zhu H, Yin X, Holley CL, Meyer KD
Front Cell Dev Biol. 2022 Apr 27;10:888279. doi: 10.3389/fcell.2022.888279. eCollection 2022. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
194402pCMV-ADARcd-YTHD422NADAR1 catalytic domain E488Q fused to YTHD422N
194431pCMV-ADARcd-YTHmutADAR1 catalytic domain E488Q fused to YTHmut
194432pCMV-APOBEC1-YTHD422NAPOBEC1 fused to YTHD422N
200158pET-APOBEC1-YTHFor bacterial expression of the APOBEC1-YTH fusion protein for protein purification
200159pET-APOBEC1-YTHmutFor bacterial expression of the APOBEC1-YTHmut fusion protein for protein purification

Antibodies from Article