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HP1alpha is a chromatin crosslinker that controls nuclear and mitotic chromosome mechanics.

Strom AR, Biggs RJ, Banigan EJ, Wang X, Chiu K, Herman C, Collado J, Yue F, Ritland Politz JC, Tait LJ, Scalzo D, Telling A, Groudine M, Brangwynne CP, Marko JF, Stephens AD
Elife. 2021 Jun 9;10. pii: 63972. doi: 10.7554/eLife.63972. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
127906Human 3' HP1a AID GFP PuroRHDR (donor) Plasmid for Inserting AID-GFP-2A-Puro into the 3' end of the Human HP1a Gene
127907pX330 Human 3' HP1a gRNAWT Cas9 Vector targeting the 3' end of the human HP1a gene
127909SB100X in pCAG globin pASleeping Beauty Transposase expression vector driven by the CAG promoter
127910pEf1a OsTIR ires NEO pA T2BHTransposon vector containing an OsTIR IRES Neo cassette driven by the Ef1a promoter

Antibodies from Article