CRISPR/Cas9 gRNA activity depends on free energy changes and on the target PAM context.
Corsi GI, Qu K, Alkan F, Pan X, Luo Y, Gorodkin J
Nat Commun. 2022 May 30;13(1):3006. doi: 10.1038/s41467-022-30515-0.
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No plasmids are currently publicly available from Addgene for this article.