Directed mutational scanning reveals a balance between acidic and hydrophobic residues in strong human activation domains.

Staller MV, Ramirez E, Kotha SR, Holehouse AS, Pappu RV, Cohen BA
Cell Syst. 2022 Apr 20;13(4):334-345.e5. doi: 10.1016/j.cels.2022.01.002. Epub 2022 Feb 3. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
176293	pMVS223	SyntheticTranscriptionFactor_loxFAS_PGK_MCS_scarlett_ERD_ZIFDBD_P2A_Neo_loxP_AMP
176294	pMVS184	Reporter_ZIFUAS_minCMVpromoter_GFP_AAVS1_Puromycin