CRISPR activation enables high-fidelity reprogramming into human pluripotent stem cells.
Sokka J, Yoshihara M, Kvist J, Laiho L, Warren A, Stadelmann C, Jouhilahti EM, Kilpinen H, Balboa D, Katayama S, Kyttala A, Kere J, Otonkoski T, Weltner J, Trokovic R
Stem Cell Reports. 2022 Feb 8;17(2):413-426. doi: 10.1016/j.stemcr.2021.12.017. Epub 2022 Jan 20.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 201677 | GG-EBNA-MIR302-3g-EEA-2g-PGK-Puro | EBNA episome plasmid for U6 promoter-driven expression of 3 gRNAs targeting miRNA302/367 (Addgene #201960) and 2 gRNAs targeting EEA-motif (Addgene #102898). Includes PGK-puro selection cassette |
| 201960 | GG-EBNA-MIR302-7guides-PGK-Puro | EBNA episome plasmid for U6 promoter-driven expression of 7 gRNAs targeting miRNA302/367. Includes PGK-puro selection cassette |