Addgene: Efficient combinatorial targeting of RNA transcripts in single cells with Cas13 RNA Perturb-seq.

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Neville Sanjana
Wessels et al

Efficient combinatorial targeting of RNA transcripts in single cells with Cas13 RNA Perturb-seq.

Wessels HH, Mendez-Mancilla A, Hao Y, Papalexi E, Mauck WM 3rd, Lu L, Morris JA, Mimitou EP, Smibert P, Sanjana NE, Satija R
Nat Methods. 2023 Jan;20(1):86-94. doi: 10.1038/s41592-022-01705-x. Epub 2022 Dec 22. (Link opens in a new window) PubMed (Link opens in a new window) Article

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ID	Plasmid	Purpose
192507	lentiGuideFB-Puro-B	For cloning of sgRNAs compatible with PspCas9. Contains a 5' direct repeat and a unique sgRNA scaffold variant with a capture sequence. Cloning guide RNAs using BsmBI.
192506	lentiGuideFB-Puro-A	For cloning of sgRNAs compatible with PspCas9. Contains a 5' direct repeat and a nd a unique sgRNA scaffold variant with a capture sequence. Cloning guide RNAs using BsmBI.
192505	pLentiRNAGuide_003	For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat and smRNA, evopreQ1, capture sequences and GFP

Antibodies from Article