Multi-pathway DNA-repair reporters reveal competition between end-joining, single-strand annealing and homologous recombination at Cas9-induced DNA double-strand breaks.

van de Kooij B, Kruswick A, van Attikum H, Yaffe MB
Nat Commun. 2022 Sep 8;13(1):5295. doi: 10.1038/s41467-022-32743-w. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
192474	pLVX_DSB_Spectrum-V1	Lentiviral DSB Spectrum V1 Reporter System
192475	pLVX_DSB_Spectrum-V2	Lentiviral DSB Spectrum V2 Reporter System
192476	pLVX_DSB_Spectrum-V3	Lentiviral DSB Spectrum V3 Reporter System
192477	pSpCas9_T2A_iRFP670 sgAAVS1	All-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1, V2, or V3 cells as a control
192478	pSpCas9_T2A_iRFP670 sgBFP	All-in-one plasmid for cutting BFP region in DSB Spectrum V2 or V3
192479	pSpCas9_T2A_mCherry sg1-sg2	All-in-one plasmid containing both guides for cutting BFP region in DSB Spectrum V1
192480	pSpCas9_T2A_mCherry sgAAVS1	All-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1 or V2 cells as control
192929	pSpCas9_T2A_mCherry sgBFP	All-in-one plasmid for cutting BFP region in DSB Spectrum V2