Stress-dependent cell stiffening by tardigrade tolerance proteins that reversibly form a filamentous network and gel.
Tanaka A, Nakano T, Watanabe K, Masuda K, Honda G, Kamata S, Yasui R, Kozuka-Hata H, Watanabe C, Chinen T, Kitagawa D, Sawai S, Oyama M, Yanagisawa M, Kunieda T
PLoS Biol. 2022 Sep 6;20(9):e3001780. doi: 10.1371/journal.pbio.3001780. eCollection 2022 Sep.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 192460 | pAcGFP1-N1-RvCAHS3-GS | Expression of RvCAHS3-GS_linker-AcGFP1 in mammalian cells |
| 192461 | pAcGFP1-N1-RvCAHS8-GS | Expression of RvCAHS8-GS_linker-AcGFP1 in mammalian cells |
| 192462 | pAcGFP1-N1-RvCAHS12-GS | Expression of RvCAHS12-GS_linker-AcGFP1 in mammalian cells |
| 192463 | pAcGFP1-N1-RvCAHS3-min-GS | Expression of RvCAHS3-min-GS_linker-AcGFP1 in mammalian cells |
| 192465 | pAcGFP1-N1-RvCAHS12-min-GS | Expression of RvCAHS12-min-GS_linker-AcGFP1 in mammalian cells |
| 192466 | pEThT-RvCAHS3 | Bacterial expression of RvCAHS3 |
| 192467 | pEThT-RvCAHS8 | Bacterial expression of RvCAHS8 |
| 192468 | pEThT-RvCAHS12 | Bacterial expression of RvCAHS12 |
| 192469 | pAc5.1/V5-HisA-RvCAHS3-GS-AcGFP1 | Transient expression of RvCAHS3 (fly codon optimized)-GS_linker-AcGFP1 in Drosophila cells |
| 192470 | pAc5.1/V5-HisA-RvCAHS8-GS-AcGFP1 | Transient expression of RvCAHS8 (fly codon optimized)-GS_linker-AcGFP1 in Drosophila cells |
| 192471 | pAc5.1/V5-HisA-RvCAHS12-GS-AcGFP1 | Transient expression of RvCAHS12 (fly codon optimized)-GS_linker-AcGFP1 in Drosophila cells |
| 192472 | pAc5-STABLE2-neo-RvCAHS3 | Stable expression of RvCAHS3 (fly codon optimized) in Drosophila cells |