Rheb regulates nuclear mTORC1 activity independent of farnesylation.
Zhong Y, Zhou X, Guan KL, Zhang J
Cell Chem Biol. 2022 Jun 16;29(6):1037-1045.e4. doi: 10.1016/j.chembiol.2022.02.006. Epub 2022 Mar 15.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 192436 | pcDNA3-mCherry-Rheb | Encodes mCherry-tagged wild-type Rheb. |
| 192437 | pcDNA3-mCherry-Rheb(C181S) | Encodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant. |
| 192438 | pcDNA3-Lyso-mCherry-Rheb(C181S) | Encodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant targeted to lysosome membrane. |
| 192439 | pcDNA3-mCherry-Rheb(5A) | Encodes mCherry-tagged Rheb lacking residues 38-42. |
| 192440 | pcDNA3-mCherry-TSC2 | Encodes mCherry-tagged wild-type TSC2 isoform 5. |
| 192441 | pcDNA3-mCherry-TSC2-NLS | Encodes mCherry-tagged wild-type TSC2 isoform 5 targeted to the nucleus. |
| 192442 | pcDNA3-mCherry-TSC2(AA) | Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation. |
| 192443 | pcDNA3-mCherry-TSC2(AA)-NLS | Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation; targeted to the nucleus. |
| 192444 | pcDNA3-mCherry-TSC2(AA,3Q) | Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A, K1614Q, K1615Q, R1616Q mutations to disrupt Akt phosphorylation and GAP activity. |
| 192445 | pcDNA3-mCherry-TSC2(AA,3Q)-NLS | Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A, K1614Q, K1615Q, R1616Q mutations to disrupt Akt phosphorylation and GAP activity; targeted to the nucleus. |