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Synthetic memory circuits for stable cell reprogramming in plants.

Lloyd JPB, Ly F, Gong P, Pflueger J, Swain T, Pflueger C, Fourie E, Khan MA, Kidd BN, Lister R
Nat Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 10.1038/s41587-022-01383-2. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
192370LLP727_L2_pV01_0I_TMV-rLUC_TCTP-fLUCTo control for variation introduced to the gene circuits, Rluc expression not affected by the introduction of terminators or recombination target sites.
192371LLP728_L2_pV01_0I_FRT-TB-rLUC_TCTP-fLUCTo test for the repressive potential of the Transcription Block (TB) with the FRT sites around it.
192372LLP729_L2_pV01_0I_FRT-OCS-rLUC_TCTP-fLUCTo test for the repressive potential of the OCS with the FRT sites around it. Then used as a control to represent the off state of many cicuits.
192373LLP730_L2_pV01_0I_FRT-OCS-TB-rLUC_TCTP-fLUCTo test for the repressive potential of the OCS and Transcription Block (TB) fusion with the FRT sites around it.
192374LLP731_L2_pV01_0I_FRT-OCStrunc-Rluc_lacZTo test for the repressive potential of the OCS variant (truncation) with the FRT sites around it.
192375LLP732_L2_pV01_0I_FRT-35Ster-Rluc_lacZTo test for the repressive potential of the 35S terminator from CaMV with the FRT sites around it.
192376LLP733_L2_pV01_0I_FRT-uORF-rLUC_TCTP-fLUCTo test for the repressive potential of the conserved peptide uORF from AT1G36730 with the FRT sites around it.
192377LLP734_L2pV1_1I_OCS-rLUC_F-NOS-FlpOTo test if the NOS promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.
192378LLP735_L2pV1_1I_OCS-rLUC_F-TCTP-FlpOTo test if the TCTP promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.
192379LLP736_L2pV1_1I_Act2_FRT_OCS_TCTP_Flp_lacZTo test if the TCTP promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output (despite the presence of the lacZ endlinker).
192380LLP737_L2pV1_1I_Act2_FRT_OCS_Act2_Flp_lacZTo test if the Act2 promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.
192381LLP738_L2pV1_1I_Act2_FRT_OCS_s35S_Flp_lacZTo test if the 35S promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.
192382LLP792_L2_FRT-OCS-tGFP_Red_mCherry_HSP-FlpOTo test if a single plasmid stably transformed into Arabidopsis can switched from off to on when heat shocked.
192383LLP739_L2_pV01_0I_B3RT-OCS-Rluc_lacZTo act as the off state for testing of the alt B3 recombinase.
192384LLP740_L2pV1_1I_B3RT-OCS-Rluc_TCTP-B3o_lacZTo act as the on state for testing of the alt B3 recombinase.
192385LLP741_L2pV1_CR_B3RT-Rluc_TCTP-FlpO_lacZTo test for if Flp is able to de-repress a circuit with B3RT, to rule out cross-reactivity between these recombinases.
192386LLP742_L2pV1_CR_FRT-Rluc_TCTP-B3o_lacZTo test for if B3 is able to de-repress a circuit with FRT, to rule out cross-reactivity between these recombinases.
192387LLP743_L2pV1_OR_B3RT_FRT_Rluc_0I_lacZTo act as the 0-input state of the OR gate.
192388LLP744_L2pV1_OR_B3RT_FRT_Rluc_1IF_lacZTo act as a 1-input state of the OR gate.
192389LLP745_L2pV1_OR_B3RT_FRT_Rluc_1IB_lacZTo act as a 1-input state of the OR gate.
192390LLP746_L2pV1_OR_B3RT_FRT_Rluc_2IFB_lacZTo act as a 2-input state of the OR gate.
192391LLP747_L2pV1_OR_B3RT_FRT_Rluc_2IBF_lacZTo act as a 2-input state of the OR gate.
192392LLP748_L2pV1_AND_B3RT_FRT_Rluc_0I_lacZTo act as the 0-input state of the AND gate.
192393LLP749_L2pV1_AND_B3RT_FRT_Rluc_1IF_lacZTo act as a 1-input state of the AND gate.
192394LLP750_L2pV1_AND_B3RT_FRT_Rluc_1IB_lacZTo act as a 1-input state of the AND gate.
192395LLP751_L2pV1_AND_B3RT_FRT_Rluc_2IFB_lacZTo act as a 2-input state of the AND gate.
192396LLP752_L2pV1_AND_B3RT_FRT_Rluc_2IBF_lacZTo act as a 2-input state of the AND gate.
192397LLP793_L2_V05_s35S_AND_GFP_pOp6_Flp_CO2_B3To test if a single plasmid stably transformed into Arabidopsis can switched from off to on when its AND gate unit is activated by both cell type (cortex) and chemical induction (by DEX).
192398LLP753_L2pV1_NOT_FRT_Rluc_0I_lacZOn state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its FRT sites.
192399LLP754_L2pV1_NOT_FRT_Rluc_1IF_lacZOff state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its FRT sites.
192400LLP755_L2pV1_NOT_B3RT_Rluc_0I_lacZOn state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its B3RT sites.
192401LLP756_L2pV1_NOT_B3RT_Rluc_1IB_lacZOff state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its B3RT sites.
192402LLP714_L2pV3_NOT_FRT_Rluc_0I_lacZOn state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its FRT sites.
192403LLP713_L2pV3_NOT_FRT_Rluc_1IF_lacZOff state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its FRT sites.
192404LLP757_L2pV3_NOT_B3RT_Rluc_0I_lacZOn state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its B3RT sites.
192405LLP758_L2pV3_NOT_B3RT_Rluc_1IB_lacZOff state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its B3RT sites.
192406LLP726_L2_pV01_0I_TMV-tGFP_TCTP-fLUCTo be a control plasmid that has no Rluc repression (replaced by turboGFP) and should reflect true off state of the circuit.
192407LLP763_L2pV3_ANIMPLYB_B3RT_FRT_OCS_Rluc_0I_lacZTo act as the 0-input state of the A NIMPLY B gate.
192408LLP764_L2pV3_ANIMPLYB_B3RT_FRT_OCS_Rluc_1IB_lacZTo act as a 1-input state of the A NIMPLY B gate.
192409LLP765_L2pV3_ANIMPLYB_B3RT_FRT_OCS_Rluc_1IF_lacZTo act as a 1-input state of the A NIMPLY B gate.
192410LLP766_L2pV3_ANIMPLYB_B3RT_FRT_OCS_Rluc_2IBF_lacZTo act as a 2-input state of the A NIMPLY B gate.
192411LLP767_L2pV3_ANIMPLYB_B3RT_FRT_OCS_Rluc_2IFB_lacZTo act as a 2-input state of the A NIMPLY B gate.
192412LLP689_L2_1IF2_FRT-OCS_Rluc_C1_FlpF2To act as a 1-input state of the split-AND gate.
192413LLP690_L2_1IF1_FRT-OCS_Rluc_FlpF1_C1-NLSTo act as a 1-input state of the split-AND gate.
192414LLP691_L2_2I_FRT-OCS_Rluc_Flp_C1To act as the 2-input state of the split-AND gate.
192415LLP715_L2sV3_NAND_1IF1_FRT_Rluc_FlpF1_C1-NLS_lacZTo act as a 1-input state of the split-NAND gate.
192416LLP716_L2sV3_NAND_1IF2_FRT_Rluc_FlpF2_C1_lacZTo act as a 1-input state of the split-NAND gate.
192417LLP717_L2sV3_NAND_2I_FRT_Rluc_Flp-C1_lacZTo act as the 2-input state of the split-NAND gate.
192418LLP759_L2pV1_OR-OCS_0ITo act as the 0-input state of the OR gate.
192419LLP760_L2pV1_OR-OCS_TCTP-FlpTo act as a 1-input state of the OR gate.
192420LLP761_L2pV1_OR-OCS_s35S-CreTo act as a 1-input state of the OR gate.
192421LLP762_L2pV1_OR-OCS_TCTP-Flp_s35S-CreTo act as the 2-input state of the OR gate.
192422LLP670_L2_pV01_0I_SloxM1-OCS-Rluc_lacZTo act as the off state for testing of the alt SCre recombinase.
192423LLP671_L2_pV01_1I_SloxM1-OCS-Rluc_TCTP_SCre_lacZTo act as the on state for testing of the alt SCre recombinase.
192424LLP672_L2_pV01_0I_VloxP-OCS-Rluc_lacZTo act as the off state for testing of the alt VCre recombinase.
192425LLP673_L2_pV01_1I_VloxP-OCS-Rluc_TCTP_VCre_lacZTo act as the on state for testing of the alt VCre recombinase.
192426LLP674_L2_pV01_0I_vox-OCS-Rluc_lacZTo act as the off state for testing of the alt Vika recombinase.
192427LLP675_L2_pV01_1I_vox-OCS-Rluc_TCTP_Vika_lacZTo act as the on state for testing of the alt Vika recombinase.
192428LLP768_L2pV1_NOR_B3RT_FRT_Rluc_0I_lacZTo act as the 0-input state of the NOR gate.
192429LLP769_L2pV1_NOR_B3RT_FRT_Rluc_1IF_lacZTo act as a 1-input state of the NOR gate.
192430LLP770_L2pV1_NOR_B3RT_FRT_Rluc_1IB_lacZTo act as a 1-input state of the NOR gate.
192431LLP771_L2pV1_NOR_B3RT_FRT_Rluc_2IFB_lacZTo act as a 2-input state of the NOR gate.
192432LLP772_L2pV1_NOR_B3RT_FRT_Rluc_2IBF_lacZTo act as a 2-input state of the NOR gate.
192433LLP720_pSP13To test the relative expression strength of the 35S promoter
192434LLP721_pSP14To test the relative expression strength of the Act2 promoter
192435LLP722_pSP15To test the relative expression strength of the TCTP1 promoter

Antibodies from Article