Enhanced production of mesencephalic dopaminergic neurons from lineage-restricted human undifferentiated stem cells.
Maimaitili M, Chen M, Febbraro F, Ucuncu E, Kelly R, Niclis JC, Christiansen JR, Mermet-Joret N, Niculescu D, Lauritsen J, Iannielli A, Klaestrup IH, Jensen UB, Qvist P, Nabavi S, Broccoli V, Nykjaer A, Romero-Ramos M, Denham M
Nat Commun. 2023 Dec 5;14(1):7871. doi: 10.1038/s41467-023-43471-0.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 192287 | pLV-hUbC-GBX2-CDX124-Cas9-T2A-GFP | Expresses Cas9-T2A-GFP and four sgRNAs: GBX2, CDX1, CDX2, CDX4 |
| 192288 | pLV-4gRNA-GBX2-RFP | Expresses RFP and four sgRNAs against GBX2 |
| 192510 | phH1-GBX2-gRNA | Contains gRNA for GBX2 |
| 192511 | phU6-CDX4-gRNA | Contains gRNA for CDX4 |
| 192512 | pmU6-CDX1-gRNA | Contains gRNA for CDX1 |
| 192513 | ph7SK-CDX2-gRNA | Contains gRNA for CDX2 |
| 192822 | pBS-BsmbI-LoxP-Ef1a-EM7-mCherry-Neo-PolyA-LoxP | Golden Gate compatible plasmid with mCherry and Neo |
| 192823 | pBS-GBX2-CDX124-Ef1a-mCherry-Neo | Expresses mCherry and four sgRNAs: GBX2, CDX1, CDX2, CDX4 |