Enhancing gene editing and gene targeting efficiencies in Arabidopsis thaliana by using an intron-containing version of ttLbCas12a.
Schindele P, Merker L, Schreiber T, Prange A, Tissier A, Puchta H
Plant Biotechnol J. 2023 Mar;21(3):457-459. doi: 10.1111/pbi.13964. Epub 2022 Nov 30.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 192350 | pAGT8162 (AATG_dttLbCas12a-i_TTCG) | L0 MoClo-compatible GoldenGate-module of dttLbCas12a-i for in planta expression |
| 192351 | pAGT5439 | L0 MoClo-compatible GoldenGate-module of the OCS terminator (tOCS) with stop |
| 192352 | pAGT9403 | L0 MoClo-compatible GoldenGate-module of the NOS terminator (tNOS) with stop |
| 192353 | pAGT10446 | L0 MoClo-compatible GoldenGate-module of the 35S terminator (t35S) with stop |
| 192453 | pAGT8163 (AATG_ttLbCas12a-i_TTCG) | intronized ttLbCas12a for in planta expression |