Comparative optimization of combinatorial CRISPR screens.
Li R, Klingbeil O, Monducci D, Young MJ, Rodriguez DJ, Bayyat Z, Dempster JM, Kesar D, Yang X, Zamanighomi M, Vakoc CR, Ito T, Sellers WR
Nat Commun. 2022 May 5;13(1):2469. doi: 10.1038/s41467-022-30196-9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 192205 | pWRS_1001 | lentiviral expression of SpCas9 and two pol III promoters for dual expression of sgRNAs |
| 192206 | pWRS3001_VCR1_WCR3 | VCR1 and WCR3 tracrRNAs cloned into pTWIST AMP high copy backbone |