Skip to main content
Addgene

A genome-scale gain-of-function CRISPR screen in CD8 T cells identifies proline metabolism as a means to enhance CAR-T therapy.

Ye L, Park JJ, Peng L, Yang Q, Chow RD, Dong MB, Lam SZ, Guo J, Tang E, Zhang Y, Wang G, Dai X, Du Y, Kim HR, Cao H, Errami Y, Clark P, Bersenev A, Montgomery RR, Chen S
Cell Metab. 2022 Apr 5;34(4):595-614.e14. doi: 10.1016/j.cmet.2022.02.009. Epub 2022 Mar 10. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
192187pSC014_pLKO-U6-BsmBI-MS2sgRNA-EFS-Thy11-2A-MS2-p65-HSF1TdgA vector
192188pLY57_TRAC-LHA-pAAV-EFS-CD22BBz-PRODH2-TRAC-RHA(3166mut)CD22 CAR AAV vector PRODH2 KI (pLY057)
192189pLY59_TRAC-LHA-pAAV-EFS-CD22BBz-PRODH2(stop)-TRAC-RHA(3166mut)CD22 CAR AAV vector PRODH2(StopCtrl) (pLY059)
192190pLY080b_AAV_EFS-4D5-CD8-28BBz (HER2 CAR)HER2 CAR AAV vector PRODH2 KI (pLY080b)
192191pLY088_AAV_EFS-BCMA-41BBz-PRODH2BCMA CAR AAV vector PRODH2 KI (pLY088)
192193pLY092b-lenti-EFS-BCMA-BBz-T2APRODH2-T2APuro-WPRELenti-BCMA-CAR-PRODH2
192194pLY093-EFS-BCMA-Blast-WPRELenti-BCMA-OE-Blast
192195pLY094-EFS-CD22-Blast-WPRELenti-CD22-OE-Blast
192196pLY095-EFS-ERBB2 HER2-Blast-WPRELenti-HER2-OE-Blast
192197pLY096-RetroEFS-CD22BBz-WPRERetro-CD22CAR
192198pLY097-RetroEFS-CD22BBz-PRODH2-WPRERetro-CD22CAR-PRODH2
192199pLY098-RetroEFS-BCMABBz-WPRERetro-BCMACAR
192200pLY099-RetroEFS-BCMABBz-PRODH2-WPRERetro-BCMACAR-PRODH2
192201pLY100-RetroEFS-HER2-28BBz-WPRERetro-HER2CAR
192202pLY101-RetroEFS-HER2-28BBz-PRODH2-WPRERetro-HER2CAR-PRODH2
192203pXD023 - pLKO-EF1a-Puro-P2A-FLuc-WPRELenti-PL(Puro-Luciferase)
192204pXD024 - pLKO-EF1a-GFP-P2A-FLuc-WPRELenti-GL(GFP-Luciferase)

Antibodies from Article