Programmable RNA sensing for cell monitoring and manipulation.
Qian Y, Li J, Zhao S, Matthews EA, Adoff M, Zhong W, An X, Yeo M, Park C, Yang X, Wang BS, Southwell DG, Huang ZJ
Nature. 2022 Oct;610(7933):713-721. doi: 10.1038/s41586-022-05280-1. Epub 2022 Oct 5.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 192063 | CAG-BFP-sesRNA-GFP-WPRE-pA | Expression of BFP, sesRNA in mammalian cells, with GFP as efRNA. |
| 192064 | pAAV-TRE3g-mNeon-WPRE-pA | mNeonGreen expression with TRE3g promoter |
| 192065 | CAG-mCherry-sesRNA-GFP-WPRE-pA | Expression of mCherry, sesRNA in mammalian cells, with GFP as efRNA. |
| 192066 | pAAV-hSyn-mCherry-sesRNA-smFlag-tTA2-W3SL | Expression of mCherry, sesRNA in mammalian cells, with smFlag and tTA2 as efRNA. |
| 192067 | pAAV-hSyn-ClipF-sesRNA-smV5-tTA2-W3SL | Expression of ClipF, sesRNA in mammalian cells, with smV5 and tTA2 as efRNA. |
| 192068 | pAAV-hSyn-ADAR2-sesRNA-tTA2-W3SL | Expression of ADAR2, sesRNA in mammalian cells with hSyn promoter, with tTA2 as efRNA and ADAR2 overexpression. |
| 192069 | CAG-ADAR2-sesRNA-GFP-W3SL | Expression of ADAR2, sesRNA in mammalian cells, with tTA2 as efRNA and ADAR2 overexpression. |
| 192070 | CAG-BFP-sesRNA-tTA2-WPRE-pA | Expression of BFP, sesRNA in mammalian cells, with tTA2 as efRNA. |