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Luca Scorrano Lab: Luca Scorrano plasmids

Scorrano L
unpublished

Plasmids from Article

ID Plasmid Purpose
191937pEGFP-N1-Letm1Expression of a Letm1 (Leucine Zipper And EF-Hand Containing Transmembrane Protein 1)- GFP chimera.
191938pcDNA3.1-Bid-GFPExpression of BID-GFP chimera
191939pMSCV-YFP-Bak-mitoExogenous expression of BAK-YFP chimera in mitochondria
191940pcDNA3.1 CalreticulinExpression of calreticulin
191941pMSCVpuro-Bax-YFPExogenous expression of a Bax-YFP chimera.
191942pECFP-Drp1Expression of a Drp1-ECFP chimera
191943pcDNA3.1-HA-Bax-mitoExpression of Bax and mitochodrial targeting sequence.
191944pcDNA3.1-HA-BaxExpression of HA-Bax.
191946pCI-His-Fis1-DTMExpression of Fis1 lacking the C-terminal 27 residues (Fis1-DTM)
191947pCI-HA-Fis1Expression of Fis1
191948pMSCV-YFP-Bak-ERExpression of a YFP- Bak chimera and endoplasmatic reticulum signal peptide.
191949pSilencer-Opa1 KOExpression of a siRNA that targets Opa1
191950pEGFP-Fis1-DTMExpression of a Fis1-GFP chimera lacking the C-terminal 27 residues (Fis1-DTM)
191951pMSCVpuro-CFP-BaxExpression of CFP-Bax chimera
191952pMSCV-Opa1Exogenous expression of OPA1
191953pcB6-Myc-Mfn2 IYFFTExpression of Mfn2 protein, in which the C-terminus, after the transmembrane domain, is replaced by a stretch of hydrophobic amino acids (IYFFT)
191954pMIMYA pMSCV-based backbone where the MCS has been substituted with a MCS-IRES-mitoYFP for expression of a gene of interest and a mitochondrially targeted YFP
191955pcDNA3.1-Mfn1Expression of Mfn1, a mediator of mitochondrial fusion.
191956pcDNA3.1-CnA-H151AExpression of phosphatase dead mutant of calcineurin A (CnA-H151A).
191957pcDNA3-PARL-D319N-FLAG C-t Expression of Presenilin Associated Rhomboid Like (PARL) D319N mutant
191958pcDNA3-PARL-D319A-FLAG C-t Expression of Presenilin Associated Rhomboid Like (PARL) D319A mutant
191959pMSCV-YFP-BakExpression of YFP-Bak chimera
191960pcDNA3.1-Mfn2Expression of the mitochondrial GTPase Mfn2.
191961pDEST-Opa1Gateway Destination Vector, containing OPA1 coding sequence, for Gateway Cloning Technology
191962pcDNA3.1-Letm1Expression of Letm1 (Leucine Zipper And EF-Hand Containing Transmembrane Protein 1).
191963pcDNA3.1-Opa1Expression of OPA1.
191964pMSCVpuro-Bcl2 Exogenous expression of Bcl2 WT
191968pcDNA-DEST47-Noa1-GFPGateway Destination Vector, containing NOA1-GFP chimera coding sequence, for Gateway Cloning Technology
191969pCDNA3.2/V5-DEST-Noa1Overexpression of Nitric Oxide Associated 1 protein (NOA1), tagged with V5.
191970pcDNA3.1-p15 Bid-KKAA-GFPExpression of the active p15 cBid KKAA mutant, chimera with GFP.
191971pMSCV2.2-IRES- GFP-Opa1Plasmid for cloning reaction, containing OPA1 coding sequence.
191972pEXP1-DEST-Noa1-HISDestination vector containing HIS-tagged NOA1 coding sequence.
191980pMSCV-Mic60Expression of V5-tagged Mic60, a component of the MICOS complex.
191981pcDNA3.1/Hygro-Mfn2-ActAExpression of Mfn2 tagged with mitochondrion localization signal (ActA)

Antibodies from Article