A continuous epistasis model for predicting growth rate given combinatorial variation in gene expression and environment.

Otto RM, Turska-Nowak A, Brown PM, Reynolds KA
Cell Syst. 2024 Feb 21;15(2):134-148.e7. doi: 10.1016/j.cels.2024.01.003. Epub 2024 Feb 9. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
191856	pCRISPR3_BC1	Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: CTTTCA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.
191857	pCRISPR3_BC2	Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: ATCATG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.
191858	pCRISPR3_BC3	Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GCATGG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.
191859	pCRISPR3_BC4	Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GTATGA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.
191860	pCRISPR3_BC5	Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: AGTCTA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.
191861	pCRISPR3_BC6	Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: CCTAGT) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.