Protocol for the transformation and engineering of edible algae Arthrospira platensis to generate heterologous protein-expressing strains.
Tabakh H, Jester BW, Zhao H, Kuestner R, Khuong N, Shanitta C, Takeuchi R, Roberts J
STAR Protoc. 2023 Feb 8;4(1):102087. doi: 10.1016/j.xpro.2023.102087.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 191823 | pDV002 | Plasmid for the transformation of the cyanobacterium Arthrospira platensis at the NS1 locus. No promoter. No terminator. Golden Gate compatible. Selection: in A. platensis: aadA; E. coli: AmpR |
| 191824 | pDV044 | Plasmid for the transformation of the cyanobacterium Arthrospira platensis at the KmR locus. pCPC600 promoter. With terminator. Golden Gate compatible. Selection: in A. platensis: KmR; E. coli: AmpR |
| 191825 | pDV052 | Plasmid for the transformation of the cyanobacterium Arthrospira platensis at the KmR locus. No promoter. No terminator. Golden Gate compatible. Selection: in A. platensis: KmR; E. coli: AmpR |