Efficient Targeted Mutagenesis in Brassica Crops Using CRISPR/Cas Systems.
Lawrenson T, Youles M, Chhetry M, Clarke M, Harwood W, Hundleby P
Methods Mol Biol. 2023;2653:253-271. doi: 10.1007/978-1-0716-3131-7_16.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 191769 | pICSL1P1_AtU626Ter26 | Cas9 guide expression in dicots |
| 191770 | pICSL1P2_AtU626Ter26 | Cas9 guide expression in dicots |
| 191771 | pICSL1P3_AtU626Ter26 | Cas9 guide expression in dicots |
| 191772 | pICSL1P4_AtU626Ter26 | Cas9 guide expression in dicots |
| 191773 | pICSL1P5_AtU626Ter26 | Cas9 guide expression in dicots |
| 191774 | pICSL1P6_AtU626Ter26 | Cas9 guide expression in dicots |
| 191775 | pICSL1P7_AtU626Ter26 | Cas9 guide expression in dicots |
| 191776 | pICSL1P1_crU626 | Cas12a guide expression in dicots |
| 191777 | pICSL1P2_crU626 | Cas12a guide expression in dicots |
| 191778 | pICSL1P3_crU626 | Cas12a guide expression in dicots |
| 191779 | pICSL1P4_crU626 | Cas12a guide expression in dicots |
| 191780 | pICSL1P5_crU626 | Cas12a guide expression in dicots |
| 191781 | pICSL1P6_crU626 | Cas12a guide expression in dicots |
| 191782 | pICSL1P7_crU626 | Cas12a guide expression in dicots |
| 191783 | pICSL11137 | Kanamycin resistance in plants |
| 191784 | pICSL11197 | SpCas9 expression in plants |
| 191785 | EC59103 | LbCas12a expression in plants |
| 191786 | pICSL4723-P1 | Level P Golden gate accepter |