Measurement of FGFR3 signaling at the cell membrane via total internal reflection fluorescence microscopy to compare the activation of FGFR3 mutants.
Hartl I, Brumovska V, Striedner Y, Yasari A, Schutz GJ, Sevcsik E, Tiemann-Boege I
J Biol Chem. 2023 Feb;299(2):102832. doi: 10.1016/j.jbc.2022.102832. Epub 2022 Dec 27.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 191752 | pcDNA3.1_mGFP-FGFR3 | Expression of N-terminally mGFP labelled wildtype FGFR3 (isoform IIIc) |
| 191753 | pcDNA3.1_mGFP-FGFR3-K508M | Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-K508M leads to inactive receptor (target mutation: c.1523A>T; silent mutation added on purpose: c.1515A>T) |
| 191754 | pcDNA3.1_mGFP-FGFR3-Y373C | Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-Y373C mutation (associated with TD1) (target mutation: c.1118A>G ; silent mutation added on purpose: c.1110C>A) |
| 191755 | pcDNA3.1_mGFP-FGFR3-G380R | Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-G380R mutation (associated with ACH; target mutation: c.1138G>A; silent mutation added on purpose: c.1131C>T) |
| 191756 | pcDNA3.1_mGFP-FGFR3-K650Q | Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-K650Q mutation (associated with HCH; target mutation: c.1948A>C; silent mutation added on purpose: c.1938C>T) |
| 191757 | pcDNA3.1_mGFP-FGFR3-K650E | Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-K650E mutation (associated with TD2; target mutation: c.1948A>G; silent mutation added on purpose: c.1938C>T) |
| 192509 | pcDNA3.1_mGFP-FGFR3-mScarlet | Expression of N-terminally mGFP and C-terminally mScarlet labelled wildtype FGFR3 (isoform IIIc) |