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A novel glutathione peroxidase-based biosensor disentangles differential subcellular accumulation of H2O2 and lipid hydroperoxides

Exposito-Rodriguez M, Reeder B, Brooke GN, Hough MA, Laissue PP, Mullineaux PM
bioRxiv 2024.01.18.576236 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
191677pICH-roGFP2-ORP1Ti plasmid pICH86988 harbouring roGFP2-ORP1 for peroxide detection in plant cells.
191680pICH-roGFP2-synORP1Ti plasmid pICH86988 harbouring roGFP2-synORP1 for H2O2 detection in plant cells.
191698pICH-roGFP2-GPXC71STi plasmid pICH86988 harbouring roGFP2-GPXC71S -a control roGFP2 fusion not responsive to peroxides used alongside plasmids 191680 and 191677
191699pICH-chl-roGFP2-ORP1Ti plasmid pICH86988 harbouring chl-roGFP2-ORP1 for peroxide detection in the plastids of plant cells.
191701pICH-chl-roGFP2-synORP1Ti plasmid pICH86988 harbouring chl-roGFP2-synORP1 for H2O2 detection in the plastids of plant cells.
191703pICH-chl-roGFP2-GPXC71STi plasmid pICH86988 harbouring roGFP2-GPXC71S -a plastid-targeted control roGFP2 fusion not responsive to peroxides used alongside plasmids 191699 and 191701
191758pICH-nuc-roGFP2-ORP1Ti plasmid pICH86988 harbouring nuc-roGFP2-ORP1 for peroxide detection in the nuclei of plant cells.
191759pICH-nuc-roGFP2-synORP1Ti plasmid pICH86988 harbouring nuc-roGFP2-synORP1 for H2O2 detection in the nuclei of plant cells.
191760pICH-nuc-roGFP2-GPXC71STi plasmid pICH86988 harbouring nuc-roGFP2-GPXC71S -a nuclear-targeted control roGFP2 fusion not responsive to peroxides used alongside plasmids 191759 and 191758

Antibodies from Article