A novel glutathione peroxidase-based biosensor disentangles differential subcellular accumulation of H2O2 and lipid hydroperoxides
Exposito-Rodriguez M, Reeder B, Brooke GN, Hough MA, Laissue PP, Mullineaux PM
bioRxiv 2024.01.18.576236
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Article
Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
191677 | pICH-roGFP2-ORP1 | Ti plasmid pICH86988 harbouring roGFP2-ORP1 for peroxide detection in plant cells. |
191680 | pICH-roGFP2-synORP1 | Ti plasmid pICH86988 harbouring roGFP2-synORP1 for H2O2 detection in plant cells. |
191698 | pICH-roGFP2-GPXC71S | Ti plasmid pICH86988 harbouring roGFP2-GPXC71S -a control roGFP2 fusion not responsive to peroxides used alongside plasmids 191680 and 191677 |
191699 | pICH-chl-roGFP2-ORP1 | Ti plasmid pICH86988 harbouring chl-roGFP2-ORP1 for peroxide detection in the plastids of plant cells. |
191701 | pICH-chl-roGFP2-synORP1 | Ti plasmid pICH86988 harbouring chl-roGFP2-synORP1 for H2O2 detection in the plastids of plant cells. |
191703 | pICH-chl-roGFP2-GPXC71S | Ti plasmid pICH86988 harbouring roGFP2-GPXC71S -a plastid-targeted control roGFP2 fusion not responsive to peroxides used alongside plasmids 191699 and 191701 |
191758 | pICH-nuc-roGFP2-ORP1 | Ti plasmid pICH86988 harbouring nuc-roGFP2-ORP1 for peroxide detection in the nuclei of plant cells. |
191759 | pICH-nuc-roGFP2-synORP1 | Ti plasmid pICH86988 harbouring nuc-roGFP2-synORP1 for H2O2 detection in the nuclei of plant cells. |
191760 | pICH-nuc-roGFP2-GPXC71S | Ti plasmid pICH86988 harbouring nuc-roGFP2-GPXC71S -a nuclear-targeted control roGFP2 fusion not responsive to peroxides used alongside plasmids 191759 and 191758 |