A green lifetime biosensor for calcium that remains bright over its full dynamic range.
van der Linden FH, Thornquist SC, ter Beek RM, Huijts JY, Hink MA, Gadella Jr. TWJ, Maimon G, Goedhart J.
bioRxiv 2024.11.05.622032; doi: https://doi.org/10.1101/2024.11.05.622032
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 191455 | pFPO-His-G-Ca-FLITS | Bacterial isolation of genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change |
| 191456 | pFPO-His-mTurquoise2_T203Y | Bacterial isolation of mTurquoise2 with a T203Y mutation |
| 191457 | pFR-mScarletI-antiFRET-P2A-G-Ca-FLITS | Compare the intensity of the genetically encoded calcium sensor (GECI) G-Ca-FLITS to mScarlet-I fluorescent protein |
| 191458 | pFR-mScarletI-antiFRET-P2A-EGFP | Compare the intensity of EGFP to mScarlet-I fluorescent protein |
| 191459 | pFR-mScarletI-antiFRET-P2A-mTurquoise2_T203Y | Compare the intensity of mTurquoise2_T203Y to mScarlet-I fluorescent protein |
| 191460 | pFR-mScarletI-antiFRET-P2A-GCaMP3 | Compare the intensity of the genetically encoded calcium sensor (GECI) GCaMP3 to mScarlet-I fluorescent protein |
| 191461 | pFR-mScarletI-antiFRET-P2A-jGCaMP7c | Compare the intensity of the genetically encoded calcium sensor (GECI) jGCaMP7c to mScarlet-I fluorescent protein |
| 191462 | 4xmts-G-Ca-FLITS | Genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change |
| 191463 | 3xnls-G-Ca-FLITS | Genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change |
| 191464 | ER-G-Ca-FLITS-KDEL | Genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change |
| 191465 | N1-G-Ca-FLITS | Genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change |
| 191466 | pFR-mTq2-antiFRET-P2A-mCherry | Compare the intensity of mCherry to mTurquoise2 fluorescent protein |
| 191467 | pFR-mTq2-antiFRET-P2A-RCaMP1h | Compare the intensity of the genetically encoded calcium sensor (GECI) RCaMP1h to mTurquoise2 fluorescent protein |
| 191468 | pFR-mTq2-antiFRET-P2A-jRCaMP1b | Compare the intensity of the genetically encoded calcium sensor (GECI) jRCaMP1b to mTurquoise2 fluorescent protein |
| 191469 | pFR-mTq2-antiFRET-P2A-K-GECO1 | Compare the intensity of the genetically encoded calcium sensor (GECI) K-GECO1 to mTurquoise2 fluorescent protein |
| 191470 | pFR-mTq2-antiFRET-P2A-CH-GECO2.1 | Compare the intensity of the genetically encoded calcium sensor (GECI) CH-GECO2.1 to mTurquoise2 fluorescent protein |
| 191471 | pFR-mTq2-antiFRET-P2A-R-GECO1 | Compare the intensity of the genetically encoded calcium sensor (GECI) R-GECO1 to mTurquoise2 fluorescent protein |
| 191472 | pDRESS-mTq2-antiFRET-P2A2-linker_noSacI | Negative control for ratiometric comparison between red fluorescent proteins and sensors |
| 191473 | pDRESS-mScarletI-antiFRET-P2A2-linker_noSacI | Negative control for ratiometric comparison between green fluorescent proteins and sensors |