Tuning of Gene Expression in Clostridium phytofermentans Using Synthetic Promoters and CRISPRi.
Rostain W, Zaplana T, Boutard M, Baum C, Tabuteau S, Sanitha M, Ramya M, Guss A, Ettwiller L, Tolonen AC
ACS Synth Biol. 2022 Dec 16;11(12):4077-4088. doi: 10.1021/acssynbio.2c00385. Epub 2022 Nov 25.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 191345 | pQmod2E-GG | Clostridium expression vector (pBP1 origin, ermR) with drop-out Plac-RFP cassette flanked with BsaI sites |
| 191346 | pQmod2S-GG | Clostridium expression vector (pBP1 origin, specR) with drop-out Plac-RFP cassette flanked with BsaI sites |
| 191347 | pQmod2C-GG | Clostridium expression vector (pBP1 origin, cmR) with drop-out Plac-RFP cassette flanked with BsaI sites |
| 191348 | pQmod3E-GG | Clostridium expression vector (pCB102 origin, ermR) with drop-out Plac-RFP cassette flanked with BsaI sites |
| 191349 | pQmod3S-GG | Clostridium expression vector (pCB102 origin, specR) with drop-out Plac-RFP cassette flanked with BsaI sites |
| 191350 | pQmod3C-GG | Clostridium expression vector (pCB102 origin, cmR) with drop-out Plac-RFP cassette flanked with BsaI sites |
| 191351 | pQmod4E-GG | Clostridium expression vector (pCD6 origin, ermR) with drop-out Plac-RFP cassette flanked with BsaI sites |
| 191352 | pATmin-GG | Clostridium expression vector (pAMB1 origin, ermR) with drop-out Plac-RFP cassette flanked with BsaI sites |
| 191354 | pQnl_Pcphy23 | Clostridium plasmid expressing NanoLuc reporter from moderate, constitutive promoter |
| 191355 | pQnl_tet | Clostridium expression vector with TetR-repressed NanoLuc expression |
| 191356 | pQdC12a | Clostridium CRISPRi plasmid with TetR-repressible dCas12a (LbCpf1) expression |