Microtubule retrograde flow retains neuronal polarization in a fluctuating state.

Schelski M, Bradke F
Sci Adv. 2022 Nov 4;8(44):eabo2336. doi: 10.1126/sciadv.abo2336. Epub 2022 Nov 4. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
191320	pBetaActin-TUBB2a-mEos3.2	Expresses fuorescently labeled beta tubulin to allow photoconversion of microtubule patches from green to red fluorescent with UV light
191321	pBetaActin-TUBB2a-Dronpa	Expresses fuorescently labeled beta tubulin to allow photoactivation of microtubule patches with very little UV light
191322	pBetaActin-mNeonGreen-CAMSAP3	Expresses the fluorescently labeled microtubule minus end marker CAMSAP3 to measure microtubule retrograde flow
191323	pBetaActin-TUBB2a-mNeonGreen	Expresses fluorescent labeled beta Tubulin to measure microtubule density
191324	pBetaActin-TUBB2a-mScarlet	Expresses fluorescent labeled beta Tubulin to measure microtubule density
191325	pBetaActin-IC2N-dCer3	Chronically inhibit dynein by expressing the dominant negative acting N-terminal domain of the dynein intermediate chain (IC2N)
191326	pBetaActin-FRB-dCer3-CAAX	Expresses the fluorescently labeled membrane anchor CAAX fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRB
191327	pBetaActin-dCer3-C2-FRB	Expresses the fluorescently labeled membrane anchor C2 fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRB
191328	pBetaActin-dCer3-C2-dCer3	Expresses the fluorescently labeled membrane anchor C2 without the chemical dimerization domain FRB as a control for recruiting proteins to plasma membrane
191329	pBetaActin-Halo-CAMSAP3	Expresses the fluorescently labeled microtubule minus end marker CAMSAP3 to measure microtubule retrograde flow
191330	pBetaActin-FKBP-mScarlet-Dync1h1motor	Expresses the fluorescently labeled dynein motor domain fused to dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB
191331	pBetaActin-FKBP-mScarlet-Dync1h1motorK2599T	Expresses the fluorescently labeled motor-deficient dynein motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB
191332	pBetaActin-FKBP-Dync1h1motor	Expresses the dynein motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB
191333	pBetaActin-FKBP-Halo-Dync1h1motor	Expresses the fluorescently labeled dynein motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB
191334	pBetaActin-FKBP-mScarlet-KIFC1motor	Expresses the fluorescently labeled minus-end directed KIFC1 motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB
191335	pBetaActin-FKBP-mScarlet-KIFC1motorN593K	Expresses the fluorescently labeled minus-end directed KIFC1 motor-deficient motor domain fused to FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB
191336	pBetaActin-KIF1aMotor-mScarlet-FKBP	Expresses the fluorescently labeled plus-end directed KIF1a motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB
191337	pBetaActin-KIF13aMotor-mScarlet-FKBP	Expresses the fluorescently labeled plus-end directed KIF13a motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB
191338	pBetaActin-TUBB2a-mEos3.2-mEos3.2-mEos3.2	Expresses triple fuorescently labeled beta tubulin to allow photoconversion of microtubule patches from green to red fluorescent with UV light in slice cultures