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The genetic landscape of a metabolic interaction.

Nguyen TN, Ingle C, Thompson S, Reynolds KA
Nat Commun. 2024 Apr 18;15(1):3351. doi: 10.1038/s41467-024-47671-0. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
191282pTETDuet_WTDHFR_WTTSBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)
191283pTETDuet_D27NDHFR_WTTSBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)
191284pTETDuet_M42FDHFR_WTTSBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)
191285pTETDuet_G121VDHFR_WTTSBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)
191286pTETDuet_M42FG121VDHFR_WTTSBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)
191287pTETDuet_F31YG121VDHFR_WTTSBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)
191288pTETDuet_WTDHFR_Q33STSBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)
191289pTETDuet_D27NDHFR_Q33STSBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)
191290pTETDuet_M42FDHFR_Q33STSBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)
191291pTETDuet_G121VDHFR_Q33STSBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)
191292pTETDuet_M42FG121VDHFR_Q33STSBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)
191293pTETDuet_F31YG121VDHFR_Q33STSBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)
191294pTETDuet_WTDHFR_R166QTSBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)
191295pTETDuet_D27NDHFR_R166QTSBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)
191296pTETDuet_M42FDHFR_R166QTSBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)
191297pTETDuet_G121VDHFR_R166QTSBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)
191298pTETDuet_M42FG121VDHFR_R166QTSBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)
191299pTETDuet_F31YG121VDHFR_R166QTSBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)
191300pTETDuet_WTDHFR_R127ATSBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)
191301pTETDuet_D27NDHFR_R127ATSBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)
191302pTETDuet_M42FDHFR_R127ATSBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)
191303pTETDuet_G121VDHFR_R127ATSBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)
191304pTETDuet_F31YG121VDHFR_R127ATSBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)
191305pTETDuet_M42FG121VDHFR_R127ATSBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)
191306pET24a_thyA_WT_untaggedBacterial expression of E. coli thyA (thymidylate synthase)
191307pET24a_thyA_R166Q_untaggedBacterial expression of E. coli thyA (thymidylate synthase)
191308pET24a_thyA_Q33S_untaggedBacterial expression of E. coli thyA (thymidylate synthase)
191309pET24a_thyA_R127A_untaggedBacterial expression of E. coli thyA (thymidylate synthase)
191310pTETDuet_F31YDHFR_WTTSBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)
191311pTETDuet_F31YDHFR_R166QTSBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)
191312pTETDuet_F31YDHFR_R127ATSBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)
191313pTETDuet_F31YDHFR_Q33STSBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Antibodies from Article