Genetic manipulation of the human gut bacterium Eggerthella lenta reveals a widespread family of transcriptional regulators.
Dong X, Guthrie BGH, Alexander M, Noecker C, Ramirez L, Glasser NR, Turnbaugh PJ, Balskus EP
Nat Commun. 2022 Dec 9;13(1):7624. doi: 10.1038/s41467-022-33576-3.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 191246 | pXD69m1-TetW | E. coli - Eggerthella lenta shuttle plasmid with tetracycline resistance in E. lenta |
| 191247 | pXD69m2-AphA | E. coli - Eggerthella lenta shuttle plasmid with kanamycin resistance in E. lenta |
| 191248 | pXD68Kan2-AphA | RSF1010-based broad host-range plasmid engineered from pAM5409, kanamycin resistance |
| 191617 | pXD70LacZ1-promoterless-LacZ | E. coli - Eggerthella lenta shuttle plasmid (KanR), promoterless-lacZ |
| 191618 | pXD70LacZ2-Pdadh-LacZ | E. coli - Eggerthella lenta shuttle plasmid (KanR), Pdadh dopamine dehydroxylase promoter-lacZ |
| 191619 | pXD70LacZ3-Pcsd-LacZ | E. coli - Eggerthella lenta shuttle plasmid (KanR), Pcsd promoter-lacZ, E. lenta constitutive promoter |
| 191620 | pXD70LacZ4-PdegV-LacZ | E. coli - Eggerthella lenta shuttle plasmid (KanR), PdegV promoter-lacZ, E. lenta constitutive promoter |
| 191621 | pXD70LacZ6-LacI-Ptac(LacO)-LacZ | E. coli - Eggerthella lenta shuttle plasmid (KanR), IPTG-inducible promoter-lacZ, engineered from pMAL-c2X |
| 191622 | pXD70LacZ7-DadR/DadS-Pdadh-LacZ | E. coli - Eggerthella lenta shuttle plasmid (KanR), Pdadh dopamine dehydroxylase promoter-lacZ with transcriptional regulators DadR/DadS |
| 191623 | pXD70LacZ8-CadR-Pcadh-LacZ | E. coli - Eggerthella lenta shuttle plasmid (KanR), Pcadh catechin dehydroxylase promoter-lacZ with transcriptional regulator CadR |
| 191624 | pXD70LacZ8.1-Pcadh-LacZ | E. coli - Eggerthella lenta shuttle plasmid (KanR), Pcadh catechin dehydroxylase promoter-lacZ |
| 191625 | pXD70LacZ9-Phcdh-LacZ | E. coli - Eggerthella lenta shuttle plasmid (KanR), Phcdh hydrocaffeic acid dehydroxylase promoter-lacZ |
| 191627 | pXD70CT5-PxylR-CymR, Pveg(CuO)-LacZ | E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5 (engineered from pCT5-bac2.0)-LacZ |
| 191628 | pXD70CT5.1-PxylR-CymR, Pveg(2CuO)-LacZ | E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1 (tandem CymR binding sites)-LacZ |
| 191629 | pXD70LacZ9.1-HcdR-Phcdh-LacZ | E. coli - Eggerthella lenta shuttle plasmid (KanR), Phcdh hydrocaffeic acid dehydroxylase promoter-lacZ with transcriptional regulator HcdR |
| 191630 | pXD70DA7-DadR/DadS-Pdadh-DadhABC(A2) | E. coli - Eggerthella lenta shuttle plasmid (KanR), native promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain with transcriptional regulators DadR/DadS |
| 191631 | pXD70DAmt1-DadR/DadS-Pdadh-DadhABC(R506S, A2) | E. coli - Eggerthella lenta shuttle plasmid (KanR), native promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain (R506S mutation) with transcriptional regulators DadR/DadS |
| 191632 | pXD70DA9-Pct5-DadhABC(A2) | E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain |
| 191633 | pXD71Cas10.0-Pct5.1-crRNA(AarI) | E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(nontargeting spacer), entry plasmid for crRNA cloning |
| 191634 | pXD71Cas10.1-Pct5.1-crRNA(dadR) | E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(dadR-targeting spacer) |
| 191635 | pXD71Cas10.1RT1-Pct5.1-crRNA(dadR)-RT(ΔdadR) | E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(dadR-targeting spacer) dadR-deleting repair template, used for dadR deletion |
| 191636 | pXD70Tet(DadR)-DadR | E. coli - Eggerthella lenta shuttle plasmid (TetR), dadR complementation |
| 191637 | pXD70Tet(DadRmt1)-DadR(ΔDBD) | E. coli - Eggerthella lenta shuttle plasmid (TetR), dadR(ΔDBD) complementation |
| 191638 | pXD70Tet(DadRmt2)-DadR(ΔTM) | E. coli - Eggerthella lenta shuttle plasmid (TetR), dadR(ΔTM) complementation |
| 191639 | pXD70Tet(DadRmt4)-DadR(linker) | E. coli - Eggerthella lenta shuttle plasmid (TetR), dadR(linker region shuffled) complementation |
| 191640 | pXD70Tet(DadR-NFLAG)-N-3xFLAG-DadR | E. coli - Eggerthella lenta shuttle plasmid (TetR), N-3xFLAG-dadR complementation |
| 191641 | pXD70Tet(DadRmt5)-N-3xFLAG-DadR(ΔDBD) | E. coli - Eggerthella lenta shuttle plasmid (TetR), N-3xFLAG-dadR(ΔDBD) complementation |
| 191642 | pXD70Tet(DadRmt6)-N-3xFLAG-DadR(ΔTM) | E. coli - Eggerthella lenta shuttle plasmid (TetR), N-3xFLAG-dadR(ΔTM) complementation |
| 191643 | pXD70Tet(DadRmt7)-N-3xFLAG-DadR(linker) | E. coli - Eggerthella lenta shuttle plasmid (TetR), N-3xFLAG-dadR(linker region shuffled) complementation |
| 191644 | pXD70Tet(DadH)-Pdadh-DadhABC(A2) | E. coli - Eggerthella lenta shuttle plasmid (TetR), native promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain |
| 191645 | pXD70Tet(DadHR)-DadR-Pdadh-DadhABC(A2) | E. coli - Eggerthella lenta shuttle plasmid (TetR), native promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain with transcriptional regulator DadR |
| 191646 | pXD71Cas10.6RT4-Pct5.1-crRNA(hcdR)-RT(ΔhcdR) | E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(hcdR-targeting spacer) hcdR-deleting repair template, used for hcdR deletion |
| 191647 | pXD71Cas10.9RT2-Pct5.1-crRNA(cadR)-RT(ΔcadR) | E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(cadR-targeting spacer) cadR-deleting repair template,used for cadR deletion |
| 191648 | pXD70Tet(HcdR)-HcdR | E. coli - Eggerthella lenta shuttle plasmid (TetR), hcdR complementation |
| 191649 | pXD70Tet(CadR)-CadR | E. coli - Eggerthella lenta shuttle plasmid (TetR), cadR complementation |
| 191650 | pXD71Cas10.4RT4-Pct5.1-crRNA(cgr2)-RT(Δcgr12) | E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(cgr2-targeting spacer) cgr1/cgr2-deleting repair template, used for cgr1/cgr2 deletion |
| 191651 | pXD71Cas10.0RTcgr-Pct5.1-crRNA(AarI)-RT(Δcgr12) | E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(nontargeting spacer) cgr1/cgr2-deleting repair template, used as negative control for pXD71Cas10.4RT4 |
| 192273 | pXD71Cas10RFP-Pct5.1-crRNA(AarI)-RFP | E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(AarI)-RFP, entry plasmid for crRNA cloning |