Thermodynamic analysis of the GAS(right) transmembrane motif supports energetic model of dimerization.
Diaz Vazquez G, Cui Q, Senes A
Biophys J. 2023 Jan 3;122(1):143-155. doi: 10.1016/j.bpj.2022.11.018. Epub 2022 Nov 12.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 191238 | pSN-A112C-GpA | Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA and a His-tag, for fluorescent studies. |
| 191239 | pSN-A112C-GpA-G83I | Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA-G83I and a His-tag, for fluorescent studies. |
| 191240 | pSN-A112C-CP8B1 | Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-CP8B1 and a His-tag, for fluorescent studies. |
| 191241 | pSN-A112C-1A32 | Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A32 and a His-tag, for fluorescent studies. |
| 191242 | pSN-A112C-1A31 | Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A31 and a His-tag, for fluorescent studies. |