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A hybrid open-top light-sheet microscope for versatile multi-scale imaging of cleared tissues.

Glaser AK, Bishop KW, Barner LA, Susaki EA, Kubota SI, Gao G, Serafin RB, Balaram P, Turschak E, Nicovich PR, Lai H, Lucas LAG, Yi Y, Nichols EK, Huang H, Reder NP, Wilson JJ, Sivakumar R, Shamskhou E, Stoltzfus CR, Wei X, Hempton AK, Pende M, Murawala P, Dodt HU, Imaizumi T, Shendure J, Beliveau BJ, Gerner MY, Xin L, Zhao H, True LD, Reid RC, Chandrashekar J, Ueda HR, Svoboda K, Liu JTC
Nat Methods. 2022 May;19(5):613-619. doi: 10.1038/s41592-022-01468-5. Epub 2022 May 11. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
191205pAAV-TRE-FRT-2xtTAFlpo dependent tTA expression under the control of TRE promotor
191206pAAV-7xTRE-3xeGFPGFP expression under the control of TRE promotor

Antibodies from Article