Highly efficient CRISPR-mediated large DNA docking and multiplexed prime editing using a single baculovirus.

Aulicino F, Pelosse M, Toelzer C, Capin J, Ilegems E, Meysami P, Rollarson R, Berggren PO, Dillingham MS, Schaffitzel C, Saleem MA, Welsh GI, Berger I
Nucleic Acids Res. 2022 Jul 22;50(13):7783-7799. doi: 10.1093/nar/gkac587. PubMed Article

Plasmids from Article

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ID	Plasmid	Purpose
206282	pMMK ENTR 2 CMV mKO2-hCdt1	ENTR Vector 2 for MultiSite Gateway assembly. Encodes mKO2-hCdt1 under the control of CMV
206281	pMMK ENTR 1 CMV mAG h-Geminin	ENTR Vector 1 for MultiSite Gateway assembly. Encodes mAG-hGeminin under the control of CMV
206280	MultiMate-Cell-cycle	Expression of a FUCCI cell cycle sensor with a H2B iRFP713 chromatin reporter. Can be used to generate recombinant baculovirus particles.
206279	pMMK ENTR 4 CMV mTagBFP	ENTR Vector 4 for MultiSite Gateway assembly. Encodes mTagBFP under the control of a CMV promoter.
206278	pMMK ENTR 3 polH aeBlue polH VSV-G	ENTR Vector 3 for MultiSite Gateway assembly. Encodes aeBlue chromoprotein and VSV-G glycoprotein under the control of viral polH promoters.
206277	pMMK ENTR 2 hU6 HEK3 PegRNA	ENTR Vector 2 for MultiSite Gateway assembly. Encodes HEK3 PegRNA
206276	pMMACE DEST CMV PE2	DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes PE2 under the control of CMV promoter. CRE-Acceptor in MultiBac system.
206274	MultiMate-PE2 HEK3	Vector for the production of recombinant baculovirus using MultiMate assembly. All-in-one vector for PE2 HEK3 prime editing. CRE-Acceptor in MultiBac system.
206273	MultiMate-PE3 HEK3	Vector for the production of recombinant baculovirus using MultiMate assembly. All-in-one vector for PE3 HEK3 prime editing.
206272	pMMK ENTR 4 CMV eGFP	ENTR Vector 4 for MultiSite Gateway assembly. Encodes eGFP under the control of a CMV promoter