Elucidating Design Principles for Engineering Cell-Derived Vesicles to Inhibit SARS-CoV-2 Infection.
Gunnels TF, Stranford DM, Mitrut RE, Kamat NP, Leonard JN
Small. 2022 May;18(19):e2200125. doi: 10.1002/smll.202200125. Epub 2022 Apr 7.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 191569 | pTFG001-ACE2(WT)-HA | ACE2 insert in a 2nd generation lentiviral transfer plasmid (pGIPZ) to generate ACE2(WT)-expressing cells. Codon-optimized for human expression. Insert contains C-terminal HA tag. |
| 191570 | pTFG002-ACE2(Mut)-HA | ACE2 insert in a 2nd generation lentiviral transfer plasmid (pGIPZ) to generate ACE2(Mut)-expressing cells. Codon-optimized for human expression. Insert contains C-terminal HA Tag. |
| 191571 | pTFG011_Spike_Parental_D614G_3xFlag | SARS-CoV-2 Spike protein under CMV with D614G, C-term 19 amino acid truncation, and 3x FLAG. For expression and pseudotyping lentiviral particles. |
| 191572 | pTFG027_Spike_sACE2-resistant_D614G_F486S_3xFlag | Mutated SARS-CoV-2 Spike under CMV with D614G, C-term 19 amino acid truncation, and 3x FLAG. Encodes F486S mutation. For expression and pseudotyping lentiviral particles. |
| 191577 | pDMS151A_Tet-On-3G | ClonTech-Takara's doxycycline-inducible, rtTA transactivator (Tet-On-3G) cloned into a 2nd generation lentiviral transfer plasmid (pGIPZ). |
| 191578 | pTFG005_Spike_Inducible_3xFlag | Inducible expression of SARS-CoV-2 Spike protein with C-terminal 3x FLAG tag under TRE3G promoter and cloned into a 2nd generation lentiviral transfer plasmid (modified pLVX with Hygro resistance). |