Expanding the eukaryotic genetic code with a biosynthesized 21st amino acid.
Wu KL, Moore JA, Miller MD, Chen Y, Lee C, Xu W, Peng Z, Duan Q, Phillips GN Jr, Uribe RA, Xiao H
Protein Sci. 2022 Oct;31(10):e4443. doi: 10.1002/pro.4443.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 191110 | pLei-sfGFP-D134* | An E. coli sfGFP reporter with one TAG at position 134 |
| 191111 | pLei-sfGFP-V2*D134* | An E. coli sfGFP reporter with two TAG at positions 2 & 134 |
| 191112 | pLei-sfGFP-V2*D134*Y152* | An E. coli sfGFP reporter with two TAG at positions 2 & 134 & 152 |
| 191113 | pLei-wt-sfGFP | An E. coli sfGFP wild type control |
| 191114 | pET22b-T5-MfnG-TEV-His | Expresses MfnG-TEV-His in E. coli |
| 191115 | pLenti-MfnG | Lentiviral vector expressing MfnG |
| 191116 | pBad-MfnG | Expresses MfnG in E. coli |
| 191117 | pCS2-wt-EGFP | An wt EGFP control for Zebrafish |
| 191118 | pCS2-EcYRS-EGFP* | A plasmid for incorporation of O-Me-Tyr into an EGFP reporter for zebrafish |
| 191119 | pCS2-MfnG-EcYRS-EGFP* | A plasmid for incorporation of O-Me-Tyr into an EGFP reporter without O-Me-Tyr feeding for zebrafish |