Prediction of efficiencies for diverse prime editing systems in multiple cell types.
Yu G, Kim HK, Park J, Kwak H, Cheong Y, Kim D, Kim J, Kim J, Kim HH
Cell. 2023 Apr 21:S0092-8674(23)00331-8. doi: 10.1016/j.cell.2023.03.034.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 191101 | pLenti-NRCH-PE2-BSD | Lentiviral expression plasmid of NRCH-PE prime editor with P2A-BSD marker |
| 191102 | pLenti-PE2max-BSD | Lentiviral expression plasmid of PEmax prime editor with P2A-BSD marker |
| 191103 | pLenti-NRCH-PE2max-BSD | Lentiviral expression plasmid of NRCH-PEmax prime editor with P2A-BSD marker |
| 191104 | pLenti-EF1a-hMLH1dn-eGFP | Lentiviral expression plasmid of hMLH1dn with P2A-eGFP reporter |
| 191105 | pLenti_gRNA -hMLHdn-Puro | Backbone for lentiviral pegRNA library construction with hMLH1dn and Puro marker |
| 195512 | pLenti-EF1a-hMLH1dn-Hygro | Lentiviral expression plasmid of hMLH1dn with P2A-HygroR marker |
| 195513 | pCMV-MMLV-gag-hMLH1dn | MMLV-Gag fused to hMLH1dn with TSTLLMENSS cleavage site between Gag-NES and hMLH1dn. For production of virus like particles with base-editor RNP cargo. |