A Simple CRISPR/Cas9 System for Efficiently Targeting Genes of Aspergillus Section Flavi Species, Aspergillus nidulans, Aspergillus fumigatus, Aspergillus terreus, and Aspergillus niger.

Chang PK
Microbiol Spectr. 2023 Feb 14;11(1):e0464822. doi: 10.1128/spectrum.04648-22. Epub 2023 Jan 18. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
191015	pAf-CRISPR-yA	CRISPR vector based on Aspergillus flavus U6 promoter and terminator for targeting the yA gene, which contains AMA1(the HindIII-PstI fragment) and ptrA selection marker.
191016	pAsp-AMA-gpdA-ptr	CRISPR vector for cloning sgDNAs to target genes of several Aspergillus species (especially section Flavi)