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The Pathfinder plasmid toolkit for genetically engineering newly isolated bacteria enables the study of Drosophila-colonizing Orbaceae.

Elston KM, Phillips LE, Leonard SP, Young E, Holley JC, Ahsanullah T, McReynolds B, Moran NA, Barrick JE
ISME Commun. 2023 May 24;3(1):49. doi: 10.1038/s43705-023-00255-3. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
190998pBTK1003 (pSL2)Expresses red chromoprotein on a broad host range RSF1010 origin plasmid
190999pBTK1004 (pSL3)Expresses red chromoprotein on a broad host range RSF1010 origin plasmid
191000pBTK1005 (pSL4)Expresses red chromoprotein on a broad host range RSF1010 origin plasmid
191001pBTK1006 (pSL5)Expresses red chromoprotein on a broad host range RSF1010 origin plasmid
191002pBTK1007 (pSL6)Expresses red chromoprotein on a broad host range RSF1010 origin plasmid
191003pBTK1008 (pSL7)Expresses red chromoprotein on a broad host range RSF1010 origin plasmid
191004pBTK1009 (pSL9)Expresses E2 Crimson on a broad host range pBBR1 origin plasmid
191005pBTK1010 (pSL25)Expresses blue chromoprotein on a broad host range RP4 origin plasmid

Antibodies from Article